Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
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ABSTRACT:
SUBMITTER: Haruki H
PROVIDER: S-EPMC3365113 | biostudies-other | 2012
REPOSITORIES: biostudies-other
Publications
Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
Haruki Hirohito H Gonzalez Monica Rengifo MR Johnsson Kai K
PloS one 20120531 5
We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for ...[more]
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