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Fabrication of glyconanoparticle microarrays.

ABSTRACT: We report a new type of microarray, based on glyconanoparticles (GNPs), to study glycan-lectin interactions. GNPs, synthesized by conjugating carbohydrate ligands on silica nanoparticles, were printed on a photoactive surface followed by covalent immobilization by light activation. The GNP microarrays could be probed by lectins labeled with fluorescein as well as fluorescein-doped silica nanoparticles (FSNPs). Results showed that FSNP as the label enhanced the signals for the higher affinity ligands than the lower ones.

SUBMITTER: Tong Q

PROVIDER: S-EPMC3405919 | biostudies-other | 2012 Apr

REPOSITORIES: biostudies-other

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Fabrication of glyconanoparticle microarrays.

Tong Qi Q Wang Xin X Wang Hui H Kubo Takuya T Yan Mingdi M

Analytical chemistry 20120312 7

We report a new type of microarray, based on glyconanoparticles (GNPs), to study glycan-lectin interactions. GNPs, synthesized by conjugating carbohydrate ligands on silica nanoparticles, were printed on a photoactive surface followed by covalent immobilization by light activation. The GNP microarrays could be probed by lectins labeled with fluorescein as well as fluorescein-doped silica nanoparticles (FSNPs). Results showed that FSNP as the label enhanced the signals for the higher affinity lig ...[more]

PMID: 22385080

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