Ontology highlight
ABSTRACT:
SUBMITTER: Devauges V
PROVIDER: S-EPMC3433432 | biostudies-other | 2012
REPOSITORIES: biostudies-other
Devauges Viviane V Marquer Catherine C Lécart Sandrine S Cossec Jack-Christophe JC Potier Marie-Claude MC Fort Emmanuel E Suhling Klaus K Lévêque-Fort Sandrine S
PloS one 20120904 9
Classical FRET (Förster Resonance Energy Transfer) using two fluorescent labels (one for the donor and another one for the acceptor) is not efficient for studying the homodimerization of a protein as only half of the homodimers formed can be identified by this technique. We thus resorted to homoFRET detected by time-resolved Fluorescence Anisotropy IMaging (tr-FAIM). To specifically image the plasma membrane of living cells, an original combination of tr-FAIM and Total Internal Reflection Fluore ...[more]