Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC3434807 | biostudies-other | 2012 Jun
REPOSITORIES: biostudies-other
Chen Jiji J Miller Andrew A Kirchmaier Ann L AL Irudayaraj Joseph M K JM
Journal of cell science 20120305 Pt 12
Although distinct epigenetic marks correlate with different chromatin states, how they are integrated within single nucleosomes to generate combinatorial signals remains largely unknown. We report the successful implementation of single molecule tools constituting fluorescence correlation spectroscopy (FCS), pulse interleave excitation-based Förster resonance energy transfer (PIE-FRET) and fluorescence lifetime imaging-based FRET (FLIM-FRET) to elucidate the composition of single nucleosomes con ...[more]