Single-shot quantitative dispersion phase microscopy.
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ABSTRACT: We present an imaging modality capable of providing high-speed optical dispersion measurements of live cells. The technique permits wide-field measurement of the optical phase shifts introduced by a sample for multiple discrete wavelengths in a single image capture. Utilizing spatial modulation and the wavelength dependence of the interference-fringe spacing, average refractive index as a function of wavelength is obtained, yielding optical dispersion measurements of the sample under observation. Because of its simple and low-cost design, the technique can be readily integrated into a standard microscope to collect additional diagnostic information about biological cells.
SUBMITTER: Lue N
PROVIDER: S-EPMC3436913 | biostudies-other | 2012 Aug
REPOSITORIES: biostudies-other
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