Ontology highlight
ABSTRACT:
SUBMITTER: Ramos L
PROVIDER: S-EPMC3449922 | biostudies-other | 2002 Jan
REPOSITORIES: biostudies-other
Ramos Luciano L Kopec Lisa A LA Sweitzer Sharon M SM Fornwald James A JA Zhao Huizhen H McAllister Paul P McNulty Dean E DE Trill John J JJ Kane James F JF
Cytotechnology 20020101 1-3
Baculovirus containing the mammalianCMV promoter, in place of the insect polyhedronpromoter (BacMam), has been used to transientlytransfect COS, CHO and CHOE1a (CHO cells expressing theE1a transcriptional activator). Using this system forthe expression of a cellular adhesion factor (SAF-3) Fcfusion protein in CHOE1a, we found that levels ofexpression were highest with a MOI of 100, 20mM sodiumbutyrate, at 34 degrees C. Production increased furtherif the cells were resuspended in fresh medium, ab ...[more]