Unknown

Dataset Information

0

Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.


ABSTRACT: Gain-of-function screens in Drosophila are an effective method with which to identify genes that affect the development of particular structures or cell types. It has been found that a fraction of 2-10% of the genes tested, depending on the particularities of the screen, results in a discernible phenotype when overexpressed. However, it is not clear to what extent a gain-of-function phenotype generated by overexpression is informative about the normal function of the gene. Thus, very few reports attempt to correlate the loss- and overexpression phenotype for collections of genes identified in gain-of-function screens. In this work we use RNA interference and in situ hybridization to annotate a collection of 123 P-GS insertions that in combination with different Gal4 drivers affect the size and/or patterning of the wing. We identify the gene causing the overexpression phenotype by expressing, in a background of overexpression, RNA interference for the genes affected by each P-GS insertion. Then, we compare the loss and gain-of-function phenotypes obtained for each gene and relate them to its expression pattern in the wing disc. We find that 52% of genes identified by their overexpression phenotype are required during normal development. However, only in 9% of the cases analyzed was there some complementarity between the gain- and loss-of-function phenotype, suggesting that, in general, the overexpression phenotypes would not be indicative of the normal requirements of the gene.

SUBMITTER: Molnar C 

PROVIDER: S-EPMC3454894 | biostudies-other | 2012 Oct

REPOSITORIES: biostudies-other

altmetric image

Publications

Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.

Molnar Cristina C   Casado Mar M   López-Varea Ana A   Cruz Cristina C   de Celis Jose F JF  

Genetics 20120713 2


Gain-of-function screens in Drosophila are an effective method with which to identify genes that affect the development of particular structures or cell types. It has been found that a fraction of 2-10% of the genes tested, depending on the particularities of the screen, results in a discernible phenotype when overexpressed. However, it is not clear to what extent a gain-of-function phenotype generated by overexpression is informative about the normal function of the gene. Thus, very few reports  ...[more]

Similar Datasets

| S-EPMC4210313 | biostudies-literature
| S-EPMC2789971 | biostudies-literature
| S-EPMC4491794 | biostudies-other
| S-EPMC8005959 | biostudies-literature
| S-EPMC2911109 | biostudies-literature
| S-EPMC4490494 | biostudies-literature
| S-EPMC4597449 | biostudies-literature
| S-EPMC6022657 | biostudies-literature
| S-EPMC6528970 | biostudies-literature
| S-EPMC4046802 | biostudies-other