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Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization.


ABSTRACT: We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni.

SUBMITTER: Dubuffet A 

PROVIDER: S-EPMC3536083 | biostudies-other | 2013 Jan

REPOSITORIES: biostudies-other

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Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization.

Dubuffet Aurore A   Smith Judith E JE   Solter Leellen L   Perotti M Alejandra MA   Braig Henk R HR   Dunn Alison M AM  

Applied and environmental microbiology 20121019 1


We designed fluorescence in situ hybridization probes for two distinct microsporidian clades and demonstrated their application in detecting, respectively, Nosema/Vairimorpha and Dictyoceola species. We used them to study the vertical transmission of two microsporidia infecting the amphipod Gammarus duebeni. ...[more]

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