Unknown

Dataset Information

0

Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei.


ABSTRACT: A tandemly arranged multigene family encoding putative hexose transporters in Trypanosoma brucei has been characterized. It is composed of two 80% homologous groups of genes called THT1 (six copies) and THT2 (five copies). When Xenopus oocytes are microinjected with in vitro-transcribed RNA from a THT1 gene, they express a glucose transporter with properties similar to those of the trypanosome bloodstream-form protein(s). This THT1-encoded transport system for glucose differs from the human erythrocyte-type glucose transporter by its moderate sensitivity to cytochalasin B and its capacity to transport D-fructose. These properties suggest that the trypanosomal transporter may be a good target for antitrypanosomal drugs. mRNA analysis revealed that expression of these genes was life cycle stage dependent. Bloodstream forms express 40-fold more THT1 than THT2. In contrast, procyclic trypanosomes express no detectable THT1 but demonstrate glucose-dependent expression of THT2.

SUBMITTER: Bringaud F 

PROVIDER: S-EPMC358999 | biostudies-other | 1993 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Differential regulation of two distinct families of glucose transporter genes in Trypanosoma brucei.

Bringaud F F   Baltz T T  

Molecular and cellular biology 19930201 2


A tandemly arranged multigene family encoding putative hexose transporters in Trypanosoma brucei has been characterized. It is composed of two 80% homologous groups of genes called THT1 (six copies) and THT2 (five copies). When Xenopus oocytes are microinjected with in vitro-transcribed RNA from a THT1 gene, they express a glucose transporter with properties similar to those of the trypanosome bloodstream-form protein(s). This THT1-encoded transport system for glucose differs from the human eryt  ...[more]

Similar Datasets

| S-EPMC1459412 | biostudies-literature
| S-EPMC1136168 | biostudies-other
| S-EPMC2863406 | biostudies-literature
| S-EPMC5078803 | biostudies-other
| S-EPMC5650466 | biostudies-literature
| S-EPMC3103310 | biostudies-literature
| S-EPMC2722254 | biostudies-literature
| S-EPMC3950673 | biostudies-literature
| S-EPMC5992589 | biostudies-literature
| S-EPMC4421009 | biostudies-literature