A Drosophila ribosomal protein functions in mammalian cells.
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ABSTRACT:
SUBMITTER: Maki CG
PROVIDER: S-EPMC361039 | biostudies-other | 1990 Sep
REPOSITORIES: biostudies-other
Publications
A Drosophila ribosomal protein functions in mammalian cells.
Maki C G CG Rhoads D D DD Diaz J J JJ Roufa D J DJ
Molecular and cellular biology 19900901 9
A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits. ...[more]
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