FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique.
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ABSTRACT:
SUBMITTER: Tai G
PROVIDER: S-EPMC3625870 | biostudies-other | 2013 Apr
REPOSITORIES: biostudies-other
Publications
FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique.
Tai Guangping G Hohenstein Peter P Davies Jamie A JA
Biology open 20130226 4
This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify th ...[more]
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