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A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter.

ABSTRACT: The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.

SUBMITTER: Lum LS

PROVIDER: S-EPMC362949 | biostudies-other | 1990 Dec

REPOSITORIES: biostudies-other

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A cloned human CCAAT-box-binding factor stimulates transcription from the human hsp70 promoter.

Lum L S LS Sultzman L A LA Kaufman R J RJ Linzer D I DI Wu B J BJ

Molecular and cellular biology 19901201 12

The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the prev ...[more]

PMID: 2247079

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