Review of microfluidic microbioreactor technology for high-throughput submerged microbiological cultivation.
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ABSTRACT: Microbial fermentation process development is pursuing a high production yield. This requires a high throughput screening and optimization of the microbial strains, which is nowadays commonly achieved by applying slow and labor-intensive submerged cultivation in shake flasks or microtiter plates. These methods are also limited towards end-point measurements, low analytical data output, and control over the fermentation process. These drawbacks could be overcome by means of scaled-down microfluidic microbioreactors (μBR) that allow for online control over cultivation data and automation, hence reducing cost and time. This review goes beyond previous work not only by providing a detailed update on the current μBR fabrication techniques but also the operation and control of μBRs is compared to large scale fermentation reactors.
SUBMITTER: Hegab HM
PROVIDER: S-EPMC3631267 | biostudies-other | 2013
REPOSITORIES: biostudies-other
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