Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs.
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ABSTRACT:
SUBMITTER: Casbon JA
PROVIDER: S-EPMC3664814 | biostudies-other | 2013 May
REPOSITORIES: biostudies-other
Publications
Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs.
Casbon James A JA Slatter Andrew F AF Musgrave-Brown Esther E Osborne Robert J RJ Lichtenstein Conrad P CP Brenner Sydney S
Nucleic acids research 20130410 10
We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers. ...[more]
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