Ontology highlight
ABSTRACT:
SUBMITTER: Guan Y
PROVIDER: S-EPMC4472016 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Guan Yinghua Y Meurer Matthias M Raghavan Sarada S Rebane Aleksander A Lindquist Jake R JR Santos Sofia S Kats Ilia I Davidson Michael W MW Mazitschek Ralph R Hughes Thomas E TE Drobizhev Mikhail M Knop Michael M Shah Jagesh V JV
Molecular biology of the cell 20150415 11
We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal fluorescent protein (mTFP1) for multiphoton, multicolor applications. Excitation of this pair by a single multiphoton excitation wavelength (MPE, 850 nm) yields well-separable emission peaks (∼120-nm separation). Using this pair, we measure homo- and hetero-oligomerization interac ...[more]