Ontology highlight
ABSTRACT:
SUBMITTER: McCallum MM
PROVIDER: S-EPMC3692575 | biostudies-other | 2013 Jul
REPOSITORIES: biostudies-other
McCallum Megan M MM Nandhikonda Premchendar P Temmer Jonathan J JJ Eyermann Charles C Simeonov Anton A Jadhav Ajit A Yasgar Adam A Maloney David D Arnold Alexander Leggy AL
Journal of biomolecular screening 20130227 6
Testing small molecules for their ability to modify cysteine residues of proteins in the early stages of drug discovery is expected to accelerate our ability to develop more selective drugs with lesser side effects. In addition, this approach also enables the rapid evaluation of the mode of binding of new drug candidates with respect to thiol reactivity and metabolism by glutathione. Herein, we describe the development of a fluorescence-based high-throughput assay that allows the identification ...[more]