Ontology highlight
ABSTRACT:
SUBMITTER: Semenov SN
PROVIDER: S-EPMC3752114 | biostudies-other | 2013 Aug
REPOSITORIES: biostudies-other
Biophysical journal 20130801 4
Delineating design principles of biological systems by reconstitution of purified components offers a platform to gauge the influence of critical physicochemical parameters on minimal biological systems of reduced complexity. Here we unravel the effect of strong reversible inhibitors on the spatiotemporal propagation of enzymatic reactions in a confined environment in vitro. We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films containing immobilized substrat ...[more]