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A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.

ABSTRACT: This study demonstrates the successful manufacture and functional characterization of alginate hydrogels containing a variety of encapsulates within polyelectrolyte multilayer-coated micropores. These microporous alginate (MPA) hydrogels are prepared via one-step internal ionotropic gelation of the alginate using polyelectrolyte multilayer-coated CaCO3 microspheres along with the weak acid glucono-?-lactone. Here, successful encapsulation of a model macromolecule and fluorescent nanoparticles within microcapsules-distributed throughout the larger alginate hydrogel-is confirmed with confocal microscopy, while the porous morphology of the MPA hydrogels is examined with scanning electron microscopy. Hydrogels constructed with uncoated CaCO3 microspheres release their contents into the surrounding environment, while those constructed with polyelectrolyte multilayer-coated CaCO3 microspheres retain the materials within the pores. MPA hydrogels containing the model enzyme glucose oxidase retained activity and are capable of reacting with small molecules from the external environment. The ability to encapsulate an assortment of functional materials within a moldable, biocompatible alginate matrix gives this approach great flexibility and potential in a wide variety of biomedical applications.

SUBMITTER: Roberts JR

PROVIDER: S-EPMC3770476 | biostudies-other | 2013

REPOSITORIES: biostudies-other

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A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.

Roberts Jason R JR Ritter Dustin W DW McShane Michael J MJ

Journal of materials chemistry. B 20130101 25

This study demonstrates the successful manufacture and functional characterization of alginate hydrogels containing a variety of encapsulates within polyelectrolyte multilayer-coated micropores. These microporous alginate (MPA) hydrogels are prepared <i>via</i> one-step internal ionotropic gelation of the alginate using polyelectrolyte multilayer-coated CaCO<sub>3</sub> microspheres along with the weak acid glucono-δ-lactone. Here, successful encapsulation of a model macromolecule and fluorescen ...[more]

PMID: 24040514

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Project description:The goal of this study was to develop and statistically optimize the metronidazole (MET), chitosan (CS) and alginate (Alg) nanoparticles (NP) nanocomposites (MET-CS-AlgNPs) using a (21 × 31 × 21) × 3 = 36 full factorial design (FFD) to investigate the effect of chitosan and alginate polymer concentrations and calcium chloride (CaCl2) concentration ondrug loading efficiency(LE), particle size and zeta potential. The concentration of CS, Alg and CaCl2 were taken as independent variables, while drug loading, particle size and zeta potential were taken as dependent variables. The study showed that the loading efficiency and particle size depend on the CS, Alg and CaCl2 concentrations, whereas zeta potential depends only on the Alg and CaCl2 concentrations. The MET-CS-AlgNPs nanocomposites were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), scanning electron microscopy (SEM) and in vitro drug release studies. XRD datashowed that the crystalline properties of MET changed to an amorphous-like pattern when the nanocomposites were formed.The XRD pattern of MET-CS-AlgNPs showed reflections at 2? = 14.2° and 22.1°, indicating that the formation of the nanocompositesprepared at the optimum conditions havea mean diameter of (165±20) nm, with a MET loading of (46.0 ± 2.1)% and a zeta potential of (-9.2 ± 0.5) mV.The FTIR data of MET-CS-AlgNPs showed some bands of MET, such as 3283, 1585 and 1413 cm-1, confirming the presence of the drug in the MET-CS-AlgNPs nanocomposites. The TGA for the optimized sample of MET-CS-AlgNPs showed a 70.2% weight loss compared to 55.3% for CS-AlgNPs, and the difference is due to the incorporation of MET in the CS-AlgNPs for the formation of MET-CS-AlgNPs nanocomposites. The release of MET from the nanocomposite showed sustained-release properties, indicating the presence of an interaction between MET and the polymer. The nanocomposite shows a smooth surface and spherical shape. The release profile of MET from its MET-CS-AlgNPs nanocomposites was found to be governed by the second kinetic model (R2 between 0.956-0.990) with more than 90% release during the first 50 h, which suggests that the release of the MET drug can be extended or prolonged via the nanocomposite formulation.

Dataset Information

A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.

Publications

A Design Full of Holes: Functional Nanofilm-Coated Microdomains in Alginate Hydrogels.

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