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Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in Aspergillus nidulans.

ABSTRACT: Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.

SUBMITTER: Yin WB 

PROVIDER: S-EPMC3795930 | biostudies-other | 2013 Nov

REPOSITORIES: biostudies-other

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Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in Aspergillus nidulans.

Yin Wen-Bing WB   Chooi Yit Heng YH   Smith Adam R AR   Cacho Ralph A RA   Hu Youcai Y   White Theodore C TC   Tang Yi Y  

ACS synthetic biology 20130611 11

Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a str  ...[more]