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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

ABSTRACT: Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC3909901 | biostudies-other | 2014

REPOSITORIES: biostudies-other

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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system.

Zhao Yicheng Y   Dai Zhen Z   Liang Yang Y   Yin Ming M   Ma Kuiying K   He Mei M   Ouyang Hongsheng H   Teng Chun-Bo CB  

Scientific reports 20140203

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells. ...[more]

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