Ontology highlight
ABSTRACT:
SUBMITTER: Yang H
PROVIDER: S-EPMC3961003 | biostudies-other | 2013 Sep
REPOSITORIES: biostudies-other
Yang Hui H Wang Haoyi H Shivalila Chikdu S CS Cheng Albert W AW Shi Linyu L Jaenisch Rudolf R
Cell 20130829 6
The type II bacterial CRISPR/Cas system is a novel genome-engineering technology with the ease of multiplexed gene targeting. Here, we created reporter and conditional mutant mice by coinjection of zygotes with Cas9 mRNA and different guide RNAs (sgRNAs) as well as DNA vectors of different sizes. Using this one-step procedure we generated mice carrying a tag or a fluorescent reporter construct in the Nanog, the Sox2, and the Oct4 gene as well as Mecp2 conditional mutant mice. In addition, using ...[more]