Ontology highlight
ABSTRACT:
SUBMITTER: Charbonnier JB
PROVIDER: S-EPMC40474 | biostudies-other | 1995 Dec
REPOSITORIES: biostudies-other
Charbonnier J B JB Carpenter E E Gigant B B Golinelli-Pimpaneau B B Eshhar Z Z Green B S BS Knossow M M
Proceedings of the National Academy of Sciences of the United States of America 19951201 25
The x-ray structure of the complex of a catalytic antibody Fab fragment with a phosphonate transition-state analog has been determined. The antibody (CNJ206) catalyzes the hydrolysis of p-nitrophenyl esters with significant rate enhancement and substrate specificity. Comparison of this structure with that of the uncomplexed Fab fragment suggests hapten-induced conformational changes: the shape of the combining site changes from a shallow groove in the uncomplexed Fab to a deep pocket where the h ...[more]