Ontology highlight
ABSTRACT:
SUBMITTER: Buskila Y
PROVIDER: S-EPMC4058870 | biostudies-other | 2014
REPOSITORIES: biostudies-other
Buskila Yossi Y Breen Paul P PP Tapson Jonathan J van Schaik André A Barton Matthew M Morley John W JW
Scientific reports 20140616
The lifespan of an acute brain slice is approximately 6-12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains b ...[more]