Orione, a web-based framework for NGS analysis in microbiology.

ABSTRACT: End-to-end next-generation sequencing microbiology data analysis requires a diversity of tools covering bacterial resequencing, de novo assembly, scaffolding, bacterial RNA-Seq, gene annotation and metagenomics. However, the construction of computational pipelines that use different software packages is difficult owing to a lack of interoperability, reproducibility and transparency. To overcome these limitations we present Orione, a Galaxy-based framework consisting of publicly available research software and specifically designed pipelines to build complex, reproducible workflows for next-generation sequencing microbiology data analysis. Enabling microbiology researchers to conduct their own custom analysis and data manipulation without software installation or programming, Orione provides new opportunities for data-intensive computational analyses in microbiology and metagenomics.Orione is available online at http://orione.crs4.it.

SUBMITTER: Cuccuru G 

PROVIDER: S-EPMC4071203 | biostudies-other | 2014 Jul

REPOSITORIES: biostudies-other