Ontology highlight
ABSTRACT:
SUBMITTER: Wang C
PROVIDER: S-EPMC4160821 | biostudies-other | 2013 Nov
REPOSITORIES: biostudies-other
Wang Chen C Chang Wei-chen WC Guo Yisong Y Huang Hui H Peck Spencer C SC Pandelia Maria E ME Lin Geng-min GM Liu Hung-wen HW Krebs Carsten C Bollinger J Martin JM
Science (New York, N.Y.) 20131010 6161
The iron-dependent epoxidase HppE converts (S)-2-hydroxypropyl-1-phosphonate (S-HPP) to the antibiotic fosfomycin [(1R,2S)-epoxypropylphosphonate] in an unusual 1,3-dehydrogenation of a secondary alcohol to an epoxide. HppE has been classified as an oxidase, with proposed mechanisms differing primarily in the identity of the O2-derived iron complex that abstracts hydrogen (H•) from C1 of S-HPP to initiate epoxide ring closure. We show here that the preferred cosubstrate is actually H2O2 and that ...[more]