Unknown

Dataset Information

0

Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.


ABSTRACT: Hypoxia-inducible factor 1 (HIF-1) is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. We show that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the Drosophila Per and Sim proteins and in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim. HIF-1 beta is a series of ARNT gene products, which can thus heterodimerize with either HIF-1 alpha or AHR. HIF-1 alpha and HIF-1 beta (ARNT) RNA and protein levels were induced in cells exposed to 1% O2 and decayed rapidly upon return of the cells to 20% O2, consistent with the role of HIF-1 as a mediator of transcriptional responses to hypoxia.

SUBMITTER: Wang GL 

PROVIDER: S-EPMC41725 | biostudies-other | 1995 Jun

REPOSITORIES: biostudies-other

altmetric image

Publications

Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.

Wang G L GL   Jiang B H BH   Rue E A EA   Semenza G L GL  

Proceedings of the National Academy of Sciences of the United States of America 19950601 12


Hypoxia-inducible factor 1 (HIF-1) is found in mammalian cells cultured under reduced O2 tension and is necessary for transcriptional activation mediated by the erythropoietin gene enhancer in hypoxic cells. We show that both HIF-1 subunits are basic-helix-loop-helix proteins containing a PAS domain, defined by its presence in the Drosophila Per and Sim proteins and in the mammalian ARNT and AHR proteins. HIF-1 alpha is most closely related to Sim. HIF-1 beta is a series of ARNT gene products, w  ...[more]

Similar Datasets

| S-EPMC307597 | biostudies-literature
| S-EPMC2258751 | biostudies-literature
| S-EPMC20401 | biostudies-literature
2008-07-27 | GSE11829 | GEO
| S-EPMC164478 | biostudies-other
| S-EPMC3894988 | biostudies-literature
2014-03-05 | E-GEOD-51121 | biostudies-arrayexpress
2014-03-05 | GSE51121 | GEO
| S-EPMC463060 | biostudies-literature
| S-EPMC116727 | biostudies-literature