Ontology highlight
ABSTRACT:
SUBMITTER: Lee YJ
PROVIDER: S-EPMC4262828 | biostudies-other | 2014
REPOSITORIES: biostudies-other
Lee Young-Jo YJ Kim Daeyoung D Lee Kihoon K Chun Jong-Yoon JY
Scientific reports 20141211
Multiplex real-time PCR with quantification of targets in a single fluorescence channel has been the demand in biotechnology industry. Here, we develop a novel analytical real-time PCR technique to detect multiple targets in a single fluorescence channel without melting curve analysis. In this technique, we show the intensity of the fluorescence signals of two discrete Tm targets is different at certain temperatures called detection temperatures, by which a high Tm target can be detected regardl ...[more]