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Parallel detection of ancient pathogens via array-based DNA capture.


ABSTRACT: DNA capture coupled with next generation sequencing is highly suitable for the study of ancient pathogens. Screening for pathogens can, however, be meticulous when assays are restricted to the enrichment of single organisms, which is common practice. Here, we report on an array-based DNA capture screening technique for the parallel detection of nearly 100 pathogens that could have potentially left behind molecular signatures in preserved ancient tissues. We demonstrate the sensitivity of our method through evaluation of its performance with a library known to harbour ancient Mycobacterium leprae DNA. This rapid and economical technique will be highly useful for the identification of historical diseases that are difficult to characterize based on archaeological information alone.

SUBMITTER: Bos KI 

PROVIDER: S-EPMC4275883 | biostudies-other | 2015 Jan

REPOSITORIES: biostudies-other

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Parallel detection of ancient pathogens via array-based DNA capture.

Bos Kirsten I KI   Jäger Günter G   Schuenemann Verena J VJ   Vågene Åshild J ÅJ   Spyrou Maria A MA   Herbig Alexander A   Nieselt Kay K   Krause Johannes J  

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20150101 1660


DNA capture coupled with next generation sequencing is highly suitable for the study of ancient pathogens. Screening for pathogens can, however, be meticulous when assays are restricted to the enrichment of single organisms, which is common practice. Here, we report on an array-based DNA capture screening technique for the parallel detection of nearly 100 pathogens that could have potentially left behind molecular signatures in preserved ancient tissues. We demonstrate the sensitivity of our met  ...[more]

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