Unknown

Dataset Information

0

Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography.


ABSTRACT: We describe an angular multiplexed imaging technique for 3-D in vivo cell tracking of sparse cell distributions and optical projection tomography (OPT) with superior time-lapse resolution and a significantly reduced light dose compared to volumetric time-lapse techniques. We demonstrate that using dual axis OPT, where two images are acquired simultaneously at different projection angles, can enable localization and tracking of features in 3-D with a time resolution equal to the camera frame rate. This is achieved with a 200x reduction in light dose compared to an equivalent volumetric time-lapse single camera OPT acquisition with 200 projection angles. We demonstrate the application of this technique to mapping the 3-D neutrophil migration pattern observed over ~25.5 minutes in a live 2 day post-fertilisation transgenic LysC:GFP zebrafish embryo following a tail wound.

SUBMITTER: Chen L 

PROVIDER: S-EPMC4399664 | biostudies-other | 2015 Apr

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3084718 | biostudies-literature
| S-EPMC3087590 | biostudies-other
| S-EPMC4550250 | biostudies-literature
| S-EPMC7907276 | biostudies-literature
| S-EPMC7329365 | biostudies-literature
| S-EPMC7400545 | biostudies-literature
| S-EPMC7881237 | biostudies-literature
| S-EPMC6401086 | biostudies-other
| S-EPMC3005161 | biostudies-other
| S-EPMC5363080 | biostudies-literature