Unknown

Dataset Information

0

Neurogranin regulates CaM dynamics at dendritic spines.


ABSTRACT: Calmodulin (CaM) plays a key role in synaptic function and plasticity due to its ability to mediate Ca(2+) signaling. Therefore, it is essential to understand the dynamics of CaM at dendritic spines. In this study we have explored CaM dynamics using live-cell confocal microscopy and fluorescence recovery after photobleaching (FRAP) to study CaM diffusion. We find that only a small fraction of CaM in dendritic spines is immobile. Furthermore, the diffusion rate of CaM was regulated by neurogranin (Ng), a CaM-binding protein enriched at dendritic spines. Interestingly, Ng did not influence the immobile fraction of CaM at recovery plateau. We have previously shown that Ng enhances synaptic strength in a CaM-dependent manner. Taken together, these data indicate that Ng-mediated enhancement of synaptic strength is due to its ability to target, rather than sequester, CaM within dendritic spines.

SUBMITTER: Petersen A 

PROVIDER: S-EPMC4471661 | biostudies-other | 2015 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4460141 | biostudies-literature
| S-EPMC5018790 | biostudies-literature
| S-EPMC3031527 | biostudies-literature
| S-EPMC6705222 | biostudies-literature
| S-EPMC5143394 | biostudies-literature
| S-EPMC5565271 | biostudies-other
| S-EPMC3340582 | biostudies-literature
| S-EPMC2981701 | biostudies-literature
| S-EPMC5222721 | biostudies-literature
| S-EPMC3244562 | biostudies-literature