Ontology highlight
ABSTRACT:
SUBMITTER: Zheng P
PROVIDER: S-EPMC4491811 | biostudies-other | 2015
REPOSITORIES: biostudies-other
Zheng Peng P Arantes Guilherme M GM Field Martin J MJ Li Hongbin H
Nature communications 20150625
Metalloproteins play indispensable roles in biology owing to the versatile chemical reactivity of metal centres. However, studying their reactivity in many metalloproteins is challenging, as protein three-dimensional structure encloses labile metal centres, thus limiting their access to reactants and impeding direct measurements. Here we demonstrate the use of single-molecule atomic force microscopy to induce partial unfolding to expose metal centres in metalloproteins to aqueous solution, thus ...[more]