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Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues.


ABSTRACT: We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.

SUBMITTER: Scoville SD 

PROVIDER: S-EPMC4519776 | biostudies-other | 2015 Jul

REPOSITORIES: biostudies-other

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