Open-top selective plane illumination microscope for conventionally mounted specimens.

ABSTRACT: We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.

SUBMITTER: McGorty R 

PROVIDER: S-EPMC4523553 | biostudies-other | 2015 Jun

REPOSITORIES: biostudies-other