Exploring the Dynamics and Mutational Landscape of Riboregulation with a Minimal Synthetic Circuit in Living Cells.
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ABSTRACT:
SUBMITTER: Rodrigo G
PROVIDER: S-EPMC4564674 | biostudies-other | 2015 Sep
REPOSITORIES: biostudies-other
Publications
Exploring the Dynamics and Mutational Landscape of Riboregulation with a Minimal Synthetic Circuit in Living Cells.
Rodrigo Guillermo G Majer Eszter E Prakash Satya S Daròs José-Antonio JA Jaramillo Alfonso A Poyatos Juan F JF
Biophysical journal 20150901 5
The regulation of gene expression, triggered by conformational changes in RNA molecules, is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design and construction of a fully synthetic riboregulatory device. We present a theoretical framework that rests on a simple energy model to predict the dynamic response of such a system. Following an equilibrium description, our framework integrates thermodynamic properties—anticipated with an RNA physic ...[more]
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