Dataset Information

Red Light Modulates Ultraviolet-Induced Gene Expression in the Epidermis of Hairless Mice.

ABSTRACT: The purpose of this study was to investigate whether low-level light therapy (LLLT) was capable of modulating expression of ultraviolet (UV) light-responsive genes in vivo.The effects of 670?nm light-emitting diode (LED) array irradiation were investigated in a hairless SHK-1 mouse epidermis model. Mice were given a single dose of UVA/UVB light, or three doses of red light (670?nm @ 8?mW/cm(2) x 312?sec, 2.5?J/cm(2) per session) spread over 24?h along with combinations of pre- and post-UV treatment with red light. Levels of 14?UV-responsive mRNAs were quantified 24?h after UV irradiation by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).The transcription of mRNAs encoding for cluster of differentiation molecule 11b (CD11b) (p?

SUBMITTER: Myakishev-Rempel M

PROVIDER: S-EPMC4605358 | biostudies-other | 2015 Oct

REPOSITORIES: biostudies-other

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Red Light Modulates Ultraviolet-Induced Gene Expression in the Epidermis of Hairless Mice.

Myakishev-Rempel Max M Stadler Istvan I Polesskaya Oksana O Motiwala Alifiya S AS Nardia Frances Barg FB Mintz Benjamin B Baranova Ancha A Zavislan James J Lanzafame Raymond J RJ

Photomedicine and laser surgery 20150923 10

<h4>Objective</h4>The purpose of this study was to investigate whether low-level light therapy (LLLT) was capable of modulating expression of ultraviolet (UV) light-responsive genes in vivo.<h4>Materials and methods</h4>The effects of 670 nm light-emitting diode (LED) array irradiation were investigated in a hairless SHK-1 mouse epidermis model. Mice were given a single dose of UVA/UVB light, or three doses of red light (670 nm @ 8 mW/cm(2) x 312 sec, 2.5 J/cm(2) per session) spread over 24 h al ...[more]

PMID: 26398729

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Project description:BackgroundEdible insects, including Oxya chinensis sinuosa Mishchenko (Oc), which is consumed as food in Asia, are considered as a human food shortage alternative, and also as a preventive measure against environmental destruction. Ultraviolet B (UVB) irradiation, which causes skin photodamage, is considered as an extrinsic skin aging factor. It reduces skin hydration, and increases wrinkle formation and reactive oxygen species (ROS) and inflammatory cytokine expression. Thus, the objective of this study was to investigate the anti-aging effects of an ethanol extract of Oc (Oc.Ex).MethodsA UVB-irradiated hairless mouse model was used to examine relevant changes in skin hydration, wrinkle formation, and skin epidermal thickness. Also, antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT) were analyzed, and Oc. Ex skin protective effects against UVB irradiation-induced photoaging were examined by determining the levels of skin hydration factors.ResultsOc.Ex improved epidermal barrier dysfunctions such as increased transepidermal water loss (TEWL) and capacitance reduction in UVB-irradiated mice. It upregulated skin hydration-related markers, including hyaluronic acid (HA), transforming growth factor (TGF)-?, and pro-collagen, in UVB-irradiated mice, compared with the vehicle control group. It also reduced UVB-induced wrinkle formation, collagen degradation, and epidermal thickness. Additionally, it remarkably suppressed the increased expression of matrix metalloproteinases (MMPs), and restored the activity of SOD and CAT in UVB-irradiated mice, compared with the vehicle control group. Furthermore, Oc. Ex treatment downregulated the production of inflammatory cytokines and phosphorylation of the mitogen-activated protein kinases (MAPKs) signaling pathway activated by UVB irradiation.ConclusionThis study revealed that Oc. Ex reduced skin thickness and the degradation of collagen fibers by increasing hydration markers and collagen-regulating factors in the skin of UVB-irradiated mice. It also inhibited UVB-induced antioxidant enzyme activity and inflammatory cytokine expression via MAPK signaling downregulation, suggesting that it prevents UVB-induced skin damage and photoaging, and has potential for clinical development in skin disease treatment.

Dataset Information

Red Light Modulates Ultraviolet-Induced Gene Expression in the Epidermis of Hairless Mice.

Publications

Red Light Modulates Ultraviolet-Induced Gene Expression in the Epidermis of Hairless Mice.

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