AID-dependent generation of resected double-strand DNA breaks and recruitment of Rad52/Rad51 in somatic hypermutation.
Ontology highlight
ABSTRACT:
SUBMITTER: Zan H
PROVIDER: S-EPMC4625537 | biostudies-other | 2003 Jun
REPOSITORIES: biostudies-other
Publications
AID-dependent generation of resected double-strand DNA breaks and recruitment of Rad52/Rad51 in somatic hypermutation.
Zan Hong H Wu Xiaoping X Komori Atsumasa A Holloman William K WK Casali Paolo P
Immunity 20030601 6
Somatic hypermutation (SHM) of immunoglobulin (Ig) genes appears to involve the generation of double-strand DNA breaks (DSBs) and their error-prone repair. Here we show that DSBs occur at a high frequency in unrearranged (germline) Ig variable (V) genes, BCL6 and c-MYC. These DSBs are blunt, target the mutational RGYW/RGY hotspot, and would be resolved through nonhomologous end-joining, as indicated by the presence of Ku70/Ku86 on these DNA ends. Upon CD40-induced expression of activation-induce ...[more]
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