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Rapid production of engineered human primary hepatocyte/fibroblast sheets.

ABSTRACT: This data article contains data related to the research article entitled "Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice," published in Biomaterials[1]. Engineered hepatocyte/fibroblast sheets (EHFSs) are used for construction of vascularized subcutaneous liver tissue without a pre-transplant vascularization procedures. Here, we described a rapid production technique of EHFSs by controlling fibroblast density and coating fetal bovine serum (FBS) onto temperature-responsive culture dishes (TRCDs). The human fibroblast monolayer formed on FBS-coated TRCDs within 1 h when seeded at a high density (at least 1.56×10(5) cells/cm(2)). The most rapid EHFS production was achieved soon after the adhesion of human primary hepatocytes onto the fibroblast layer.

SUBMITTER: Sakai Y

PROVIDER: S-EPMC4631890 | biostudies-other | 2015 Dec

REPOSITORIES: biostudies-other

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Rapid production of engineered human primary hepatocyte/fibroblast sheets.

Sakai Yusuke Y Koike Makiko M Soyama Akihiko A Hidaka Masaaki M Kuroki Tamotsu T Eguchi Susumu S

Data in brief 20151009

This data article contains data related to the research article entitled "Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice," published in Biomaterials[1]. Engineered hepatocyte/fibroblast sheets (EHFSs) are used for construction of vascularized subcutaneous liver tissue without a pre-transplant vascularization procedures. Here, we described a rapid production technique of EHFSs by controlling fibroblast density and coating fetal bovine serum (FBS) ...[more]

PMID: 26629493

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