Ontology highlight
ABSTRACT:
SUBMITTER: Hannan NR
PROVIDER: S-EPMC3673228 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Hannan Nicholas R F NR Segeritz Charis-Patricia CP Touboul Thomas T Vallier Ludovic L
Nature protocols 20130201 2
Large-scale production of hepatocytes from a variety of genetic backgrounds would be beneficial for drug screening and to provide a source of cells to be used as a substitute for liver transplantation. However, fully functional primary hepatocytes remain difficult to expand in vitro, and circumventing this problem by using an alternative source of cells is desirable. Here we describe a 25-d protocol to direct the differentiation of human pluripotent stem cells into a near-homogenous population o ...[more]