Ontology highlight
ABSTRACT:
SUBMITTER: Frommer WB
PROVIDER: S-EPMC46843 | biostudies-other | 1993 Jul
REPOSITORIES: biostudies-other
Frommer W B WB Hummel S S Riesmeier J W JW
Proceedings of the National Academy of Sciences of the United States of America 19930701 13
To study amino acid transport in plants at the molecular level, we have isolated an amino acid permease cDNA from Arabidopsis thaliana by complementation of a yeast mutant defective in proline uptake with a cDNA. The predicted polypeptide of 53 kDa is highly hydrophobic with 12 putative membrane-spanning regions and shows no significant homologies to other known transporters. Expression of the cDNA enables the yeast mutant to take up L-[14C]proline. Competition studies argue for a broad but ster ...[more]