Unknown

Dataset Information

0

Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6.

ABSTRACT: Eukaryotic translation initiation factor 6 (eIF6) binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. In this paper, we devised a procedure for purifying eIF6 from rabbit reticulocyte lysates and immunochemically characterized the protein by using antibodies isolated from egg yolks of laying hens immunized with rabbit eIF6. By using these monospecific antibodies, a 1.096-kb human cDNA that encodes an eIF6 of 245 amino acids (calculated Mr 26,558) has been cloned and expressed in Escherichia coli. The purified recombinant human protein exhibits biochemical properties that are similar to eIF6 isolated from mammalian cell extracts. Database searches identified amino acid sequences from Saccharomyces cerevisiae, Drosophila, and the nematode Caenorhabditis elegans with significant identity to the deduced amino acid sequence of human eIF6, suggesting the presence of homologues of human eIF6 in these organisms.

SUBMITTER: Si K 

PROVIDER: S-EPMC24943 | biostudies-literature | 1997 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6.

Si K K   Chaudhuri J J   Chevesich J J   Maitra U U  

Proceedings of the National Academy of Sciences of the United States of America 19971201 26

Eukaryotic translation initiation factor 6 (eIF6) binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. In this paper, we devised a procedure for purifying eIF6 from rabbit reticulocyte lysates and immunochemically characterized the protein by using antibodies isolated from egg yolks of laying hens immunized with rabbit eIF6. By using these monospecific antibodies, a 1.096-kb human cDNA that encodes an eIF6 of 245 amino acids (calculated Mr 26,558) has b  ...[more]

Similar Datasets

Unknown Molecular cloning and expression of cDNA for mammalian translation initiation factor 5.
| S-EPMC46236 | biostudies-other
Unknown Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase.
| S-EPMC21548 | biostudies-literature
Unknown Molecular cloning and functional expression of chitinase-encoding cDNA from the cabbage moth, Mamestra brassicae.
| S-EPMC3887735 | biostudies-literature
Unknown Molecular cloning, sequencing, and functional expression of a cDNA encoding human coproporphyrinogen oxidase.
| S-EPMC43507 | biostudies-other
Unknown Cloning, expression, and characterization of a cDNA encoding a novel human growth factor for primitive hematopoietic progenitor cells.
| S-EPMC23864 | biostudies-literature
Unknown Molecular cloning and expression of the mouse translation initiation factor eIF-1A.
| S-EPMC147881 | biostudies-other
Unknown Molecular cloning of cDNA encoding the small subunit of Drosophila transcription initiation factor TFIIF.
| S-EPMC306958 | biostudies-other
Unknown Functional expression of a cDNA encoding a human ecto-ATPase.
| S-EPMC1571647 | biostudies-literature
Unknown A novel functional human eukaryotic translation initiation factor 4G.
| S-EPMC121501 | biostudies-literature
Unknown Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information.
| S-EPMC1217213 | biostudies-literature