Endogenous TRIM5? Function Is Regulated by SUMOylation and Nuclear Sequestration for Efficient Innate Sensing in Dendritic Cells.

ABSTRACT: During retroviral infection, viral capsids are subject to restriction by the cellular factor TRIM5?. Here, we show that dendritic cells (DCs) derived from human and non-human primate species lack efficient TRIM5?-mediated retroviral restriction. In DCs, endogenous TRIM5? accumulates in nuclear bodies (NB) that partly co-localize with Cajal bodies in a SUMOylation-dependent manner. Nuclear sequestration of TRIM5? allowed potent induction of type I interferon (IFN) responses during infection, mediated by sensing of reverse transcribed DNA by cGAS. Overexpression of TRIM5? or treatment with the SUMOylation inhibitor ginkgolic acid (GA) resulted in enforced cytoplasmic TRIM5? expression and restored efficient viral restriction but abrogated type I IFN production following infection. Our results suggest that there is an evolutionary trade-off specific to DCs in which restriction is minimized to maximize sensing. TRIM5? regulation via SUMOylation-dependent nuclear sequestration adds to our understanding of how restriction factors are regulated.

SUBMITTER: Portilho DM 

PROVIDER: S-EPMC4713866 | biostudies-other | 2016 Jan

REPOSITORIES: biostudies-other