Threshold changes in rat brain docosahexaenoic acid incorporation and concentration following graded reductions in dietary alpha-linolenic acid.

ABSTRACT: This study tested the dietary level of alpha-linolenic acid (?-LNA, 18:3n-3) required to maintain brain (14)C-Docosahexaenoic acid (DHA, 22:6n-3) metabolism and concentration following graded ?-LNA reduction.Fischer-344 (CDF) male rat pups (18-21 days old) were randomized to the AIN-93G diet containing as a % of total fatty acids, 4.6% ("n-3 adequate"), 3.6%, 2.7%, 0.9% or 0.2% ("n-3 deficient") ?-LNA for 15 weeks. Rats were intravenously infused with (14)C-DHA to steady state for 5 min, serial blood samples collected to obtain plasma, and brains excised following microwave fixation. Labeled and unlabeled DHA concentrations were measured in plasma and brain to calculate the incorporation coefficient, k*, and incorporation rate, J(in).Compared to 4.6% ?-LNA controls, k* was significantly increased in ethanolamine glycerophospholipids in the 0.2% ?-LNA group. Circulating unesterified DHA and brain incorporation rates (J(in)) were significantly reduced at 0.2% ?-LNA. Brain total lipid and phospholipid DHA concentrations were reduced at or below 0.9% ?-LNA.Threshold changes for brain DHA metabolism and concentration were maintained at or below 0.9% dietary ?-LNA, suggesting the presence of homeostatic mechanisms to maintain brain DHA metabolism when dietary ?-LNA intake is low.

SUBMITTER: Taha AY 

PROVIDER: S-EPMC4752724 | biostudies-other | 2016 Feb

REPOSITORIES: biostudies-other