Unknown

Dataset Information

0

Non-invasive cell type selective in vivo monitoring of insulin resistance dynamics.


ABSTRACT: Insulin resistance contributes to the development of cardio-vascular disease and diabetes. An important but unresolved task is to study the dynamics of insulin resistance in selective cell types of insulin target tissues in vivo. Here we present a novel technique to monitor insulin resistance dynamics non-invasively and longitudinally in vivo in a cell type-specific manner, exemplified by the pancreatic ?-cell situated within the micro-organ the islet of Langerhans. We utilize the anterior chamber of the eye (ACE) as a transplantation site and the cornea as a natural body-window to study the development and reversibility of insulin resistance. Engrafted islets in the ACE that express a FoxO1-GFP-based biosensor in their ?-cells, report on insulin resistance measured by fluorescence microscopy at single-cell resolution in the living mouse. This technique allows monitoring of cell type specific insulin sensitivity/resistance in real-time in the context of whole body insulin resistance during progression and intervention of disease.

SUBMITTER: Paschen M 

PROVIDER: S-EPMC4761884 | biostudies-other | 2016 Feb

REPOSITORIES: biostudies-other

altmetric image

Publications

Non-invasive cell type selective in vivo monitoring of insulin resistance dynamics.

Paschen Meike M   Moede Tilo T   Leibiger Barbara B   Jacob Stefan S   Bryzgalova Galyna G   Leibiger Ingo B IB   Berggren Per-Olof PO  

Scientific reports 20160222


Insulin resistance contributes to the development of cardio-vascular disease and diabetes. An important but unresolved task is to study the dynamics of insulin resistance in selective cell types of insulin target tissues in vivo. Here we present a novel technique to monitor insulin resistance dynamics non-invasively and longitudinally in vivo in a cell type-specific manner, exemplified by the pancreatic β-cell situated within the micro-organ the islet of Langerhans. We utilize the anterior chamb  ...[more]

Similar Datasets

| S-EPMC5794967 | biostudies-literature
2024-01-15 | GSE245944 | GEO
| S-EPMC5678123 | biostudies-literature
| S-EPMC7904581 | biostudies-literature
| S-EPMC8275772 | biostudies-literature
| S-EPMC7904828 | biostudies-literature
| S-EPMC6172211 | biostudies-literature
2020-12-07 | PXD021602 | Pride
| S-EPMC5817116 | biostudies-literature
| S-EPMC5773698 | biostudies-literature