Purification, Visualization, and Molecular Signature of Neural Stem Cells.
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ABSTRACT:
SUBMITTER: Yu YH
PROVIDER: S-EPMC4770853 | biostudies-other | 2016 Jan
REPOSITORIES: biostudies-other
Publications
Purification, Visualization, and Molecular Signature of Neural Stem Cells.
Yu Yuan Hong YH Narayanan Gunaseelan G Sankaran Shvetha S Ramasamy Srinivas S Chan Shi Yu SY Lin Shuping S Chen Jinmiao J Yang Henry H Srivats Hariharan H Ahmed Sohail S
Stem cells and development 20151116 2
Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1 as a novel marker for NSCs and show that it can be used along with Lewis-X (LeX) to yield a highly purified population of NSCs. Using time-lapse microscopy, we are able to follow NSCs forming neurospheres, all ...[more]
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