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Dataset reporting BCKDK interference in a BCAA-catabolism restricted environment.


ABSTRACT: This data article contains complementary figures to the research article "Mitochondrial response to the BCKDK-deficiency: some clues to understand the positive dietary response in this form of autism" [1]. Herein we present data relative to the effect of knocking down BCKDK gene on the real time oxygen consumption rate of fibroblasts obtained from a Maple Syrup Urine Disease (MSUD) patient. Interference of BCKDK expression on such cells showing a reduced branched-chain ?-ketoacid dehydrogenase (BCKDHc) activity; let us generate a scenario to study the direct effect of BCKDK absence in an environment of high branched-chain amino acids (BCAAs) concentrations. Data relative to the effectiveness of the knockdown together with the potentiality of the BCKDK-knockdown to increase the deficient branched-chain ?-ketoacid dehydrogenase activity detected in MSUD patients are also shown.

SUBMITTER: Bravo-Alonso I 

PROVIDER: S-EPMC4804226 | biostudies-other | 2016 Jun

REPOSITORIES: biostudies-other

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Dataset reporting BCKDK interference in a BCAA-catabolism restricted environment.

Bravo-Alonso I I   Oyarzabal A A   Sánchez-Aragó M M   Rejas M T MT   Merinero B B   García-Cazorla A A   Artuch R R   Ugarte M M   Rodríguez-Pombo P P  

Data in brief 20160315


This data article contains complementary figures to the research article "Mitochondrial response to the BCKDK-deficiency: some clues to understand the positive dietary response in this form of autism" [1]. Herein we present data relative to the effect of knocking down BCKDK gene on the real time oxygen consumption rate of fibroblasts obtained from a Maple Syrup Urine Disease (MSUD) patient. Interference of BCKDK expression on such cells showing a reduced branched-chain α-ketoacid dehydrogenase (  ...[more]

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