Ontology highlight
ABSTRACT:
SUBMITTER: Robitaille K
PROVIDER: S-EPMC4813485 | biostudies-other | 2016 Feb
REPOSITORIES: biostudies-other
Robitaille Karine K Rourke Jillian L JL McBane Joanne E JE Fu Accalia A Baird Stephen S Du Qiujiang Q Kin Tatsuya T Shapiro A M James AM Screaton Robert A RA
The Journal of biological chemistry 20160106 9
The expansion of cells for regenerative therapy will require the genetic dissection of complex regulatory mechanisms governing the proliferation of non-transformed human cells. Here, we report the development of a high-throughput RNAi screening strategy specifically for use in primary cells and demonstrate that silencing the cell cycle-dependent kinase inhibitors CDKN2C/p18 or CDKN1A/p21 facilitates cell cycle entry of quiescent adult human pancreatic beta cells. This work identifies p18 and p21 ...[more]