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Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle.


ABSTRACT: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies.Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N?=?260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P?

SUBMITTER: Goszczynski DE 

PROVIDER: S-EPMC4818460 | biostudies-other | 2016

REPOSITORIES: biostudies-other

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Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle.

Goszczynski Daniel Estanislao DE   Mazzucco Juliana Papaleo JP   Ripoli María Verónica MV   Villarreal Edgardo Leopoldo EL   Rogberg-Muñoz Andrés A   Mezzadra Carlos Alberto CA   Melucci Lilia Magdalena LM   Giovambattista Guillermo G  

Journal of animal science and technology 20160401


<h4>Background</h4>Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs)  ...[more]

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