Engineering Escherichia coli for Microbial Production of Butanone.
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ABSTRACT:
SUBMITTER: Srirangan K
PROVIDER: S-EPMC4836414 | biostudies-other | 2016 May
REPOSITORIES: biostudies-other
Publications
Engineering Escherichia coli for Microbial Production of Butanone.
Srirangan Kajan K Liu Xuejia X Akawi Lamees L Bruder Mark M Moo-Young Murray M Chou C Perry CP
Applied and environmental microbiology 20160418 9
To expand the chemical and molecular diversity of biotransformation using whole-cell biocatalysts, we genetically engineered a pathway in Escherichia coli for heterologous production of butanone, an important commodity ketone. First, a 1-propanol-producing E. coli host strain with its sleeping beauty mutase (Sbm) operon being activated was used to increase the pool of propionyl-coenzyme A (propionyl-CoA). Subsequently, molecular heterofusion of propionyl-CoA and acetyl-CoA was conducted to yield ...[more]
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