Yeast V-ATPase Proteolipid Ring Acts as a Large-conductance Transmembrane Protein Pore.
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ABSTRACT:
SUBMITTER: Couoh-Cardel S
PROVIDER: S-EPMC4838861 | biostudies-other | 2016 Apr
REPOSITORIES: biostudies-other
Publications
Yeast V-ATPase Proteolipid Ring Acts as a Large-conductance Transmembrane Protein Pore.
Couoh-Cardel Sergio S Hsueh Yi-Ching YC Wilkens Stephan S Movileanu Liviu L
Scientific reports 20160421
The vacuolar H(+)-ATPase (V-ATPase) is a rotary motor enzyme that acidifies intracellular organelles and the extracellular milieu in some tissues. Besides its canonical proton-pumping function, V-ATPase's membrane sector, Vo, has been implicated in non-canonical functions including membrane fusion and neurotransmitter release. Here, we report purification and biophysical characterization of yeast V-ATPase c subunit ring (c-ring) using electron microscopy and single-molecule electrophysiology. We ...[more]
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