Ontology highlight
ABSTRACT:
SUBMITTER: Strasser B
PROVIDER: S-EPMC3199395 | biostudies-literature | 2011 Oct
REPOSITORIES: biostudies-literature
Strasser Bernd B Iwaszkiewicz Justyna J Michielin Olivier O Mayer Andreas A
The EMBO journal 20110920 20
The V-ATPase V(0) sector associates with the peripheral V(1) sector to form a proton pump. V(0) alone has an additional function, facilitating membrane fusion in the endocytic and late exocytic pathways. V(0) contains a hexameric proteolipid cylinder, which might support fusion as proposed in proteinaceous pore models. To test this, we randomly mutagenized proteolipids. We recovered alleles that preserve proton translocation, normal SNARE activation and trans-SNARE pairing but that impair lipid ...[more]